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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/10280

Title: Electrochemical assay for saccharide-protein interactions using glycopolymer-modified gold nanoparticles
Authors: Ishii, Jin
Chikae, Miyuki
Toyoshima, Masayuki
Ukita, Yoshiaki
Miura, Yoshiko
Takamura, Yuzuru
Keywords: Electrochemical assay
gold nanoparticles
saccharide-protein interactions
Issue Date: 2011
Publisher: Elsevier
Magazine name: Electrochemistry Communications
Volume: 13
Number: 8
Start page: 830
End page: 833
DOI: 10.1016/j.elecom.2011.05.014
Abstract: A novel recognition element, made of electroactive glycopolymer-modified gold nanoparticles (GM-GNPs), was synthesized and used in an electrochemical assay of saccharide-protein interactions for the detection of concanavalin A (Con A). The electroactivity of the mannose-based GM-GNPs was investigated, and an electrochemical Con A assay system with a disposable screen printed carbon strips was proposed. A complex containing GM-GNPs was formed by a recognition reaction between the mannose-based glycopolymer and Con A. The complex was detected by the pre-oxidation of the GNPs and following reduction scans using differential pulse voltammetry (DPV). Under optimal conditions, a linear relationship between the DPV peak current intensity and Con A concentration was shown within an analytical range of 10-10,000 ng/mL. Our proposed new electric approach provides an interesting tool for the analysis of saccharide-protein interactions.
Rights: NOTICE: This is the author's version of a work accepted for publication by Elsevier. Jin Ishii, Miyuki Chikae, Masayuki Toyoshima, Yoshiaki Ukita, Yoshiko Miura, Yuzuru Takamura, Electrochemistry Communications, 13(8), 2011, 830-833, http://dx.doi.org/10.1016/j.elecom.2011.05.014
URI: http://hdl.handle.net/10119/10280
Material Type: author
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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