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http://hdl.handle.net/10119/10280
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| Title: | Electrochemical assay for saccharide-protein interactions using glycopolymer-modified gold nanoparticles |
| Authors: | Ishii, Jin
Chikae, Miyuki
Toyoshima, Masayuki
Ukita, Yoshiaki
Miura, Yoshiko
Takamura, Yuzuru |
| Keywords: | Electrochemical assay
mannose
glycopolymer
gold nanoparticles
saccharide-protein interactions |
| Issue Date: | 2011 |
| Publisher: | Elsevier |
| Magazine name: | Electrochemistry Communications |
| Volume: | 13 |
| Number: | 8 |
| Start page: | 830 |
| End page: | 833 |
| DOI: | 10.1016/j.elecom.2011.05.014 |
| Abstract: | A novel recognition element, made of electroactive glycopolymer-modified gold nanoparticles (GM-GNPs), was synthesized and used in an electrochemical assay of saccharide-protein interactions for the detection of concanavalin A (Con A). The electroactivity of the mannose-based GM-GNPs was investigated, and an electrochemical Con A assay system with a disposable screen printed carbon strips was proposed. A complex containing GM-GNPs was formed by a recognition reaction between the mannose-based glycopolymer and Con A. The complex was detected by the pre-oxidation of the GNPs and following reduction scans using differential pulse voltammetry (DPV). Under optimal conditions, a linear relationship between the DPV peak current intensity and Con A concentration was shown within an analytical range of 10-10,000 ng/mL. Our proposed new electric approach provides an interesting tool for the analysis of saccharide-protein interactions. |
| Rights: | NOTICE: This is the author's version of a work accepted for publication by Elsevier. Jin Ishii, Miyuki Chikae, Masayuki Toyoshima, Yoshiaki Ukita, Yoshiko Miura, Yuzuru Takamura, Electrochemistry Communications, 13(8), 2011, 830-833, http://dx.doi.org/10.1016/j.elecom.2011.05.014 |
| URI: | http://hdl.handle.net/10119/10280 |
| Material Type: | author |
| Appears in Collections: | c10-1. 雑誌掲載論文 (Journal Articles)
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