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http://hdl.handle.net/10119/12086
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| Title: | Cryoprotective properties of completely synthetic polyampholytes via reversible addition-fragmentation chain transfer (RAFT) polymerization and the effects of hydrophobicity |
| Authors: | Rajan, Robin
Jain, Minkle
Matsumura, Kazuaki |
| Keywords: | RAFT polymerization
polyampholyte
biocompatibility
cryopreservation |
| Issue Date: | 2013-05-30 |
| Publisher: | Taylor & Francis |
| Magazine name: | Journal of Biomaterials Science, Polymer Edition |
| Volume: | 24 |
| Number: | 15 |
| Start page: | 1767 |
| End page: | 1780 |
| DOI: | 10.1080/09205063.2013.801703 |
| Abstract: | A completely synthetic polyampholyte cryoprotectant was developed with cationic and anionic monomers by reversible addition-fragmentation chain transfer polymerization. The neutralized random polyampholyte, which had an equal composition ratio of monomers, showed high cryoprotective properties in mammalian cells. Introduction of a small amount of hydrophobic monomer enhanced cell viability after cryopreservation, indicating the importance of hydrophobicity. Leakage experiments confirmed that these polyampholytes protected the cell membrane during cryopreservation. Due to low cytotoxicity, this polyampholyte has the potential to replace the convention cryoprotective agent dimethyl sulfoxide. The present study is the first to show that we can design a polymeric cryoprotectant that will protect the cell membrane during freezing using appropriate polymerization techniques. |
| Rights: | This is an Author's Accepted Manuscript of an article published in Journal of Biomaterials Science, Polymer Edition, 24(15), 2013, 1767-1780. Copyright (C) 2013 Taylor & Francis, available online at: http://www.tandfonline.com/10.1080/09205063.2013.801703 |
| URI: | http://hdl.handle.net/10119/12086 |
| Material Type: | author |
| Appears in Collections: | c10-1. 雑誌掲載論文 (Journal Articles)
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