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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/12245

Title: Protein Cytoplasmic Delivery using Polyampholyte Nanoparticles and Freeze Concentration
Authors: Ahmed, Sana
Hayashi, Fumiaki
Nagashima, Toshio
Matsumura, Kazuaki
Keywords: Protein delivery
Freeze concentration
Issue Date: 2014-05-10
Publisher: Elsevier
Magazine name: Biomaterials
Volume: 35
Number: 24
Start page: 6508
End page: 6518
DOI: 10.1016/j.biomaterials.2014.04.030
Abstract: A protein delivery method using freeze concentration was presented with a variety of polyampholyte nanocarriers. In order to develop protein nanocarriers, hydrophobically modified polyampholytes were synthesized by the succinylation of ε-poly-L-lysine with dodecyl succinic anhydride and succinic anhydride. The self-assembled polyampholyte aggregated form nanoparticles through intermolecular hydrophobic and electrostatic interactions when dissolved in aqueous media. The cationic and anionic nanoparticles were easily prepared by changing the succinylation ratio. Anionic or cationic proteins were adsorbed on/into the nanoparticles depending on their surface charges. The protein-loaded nanoparticles were stable for at least 7 d. When L929 cells were frozen with the protein-loaded nanoparticles in the presence of a cryoprotectant, the adsorption of the protein-loaded nanoparticles was enhanced and can be explained by the freeze concentration mechanism. After thawing, proteins were internalized into cells via endocytosis. This was the first report that showed that the efficacy of protein delivery was successfully enhanced by the freeze concentration method. This method could be useful for in vitro cytoplasmic protein or peptide delivery to various cells for immunotherapy or phenotype transformations.
Rights: NOTICE: This is the author's version of a work accepted for publication by Elsevier. Sana Ahmed, Fumiaki Hayashi, Toshio Nagashima, Kazuaki Matsumura, Biomaterials, 35(24), 2014, 6508-6518, http://dx.doi.org/10.1016/j.biomaterials.2014.04.030
URI: http://hdl.handle.net/10119/12245
Material Type: author
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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