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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/12326

Title: Efficient Production of Live Offspring from Mouse Oocytes Vitrified with a Novel Cryoprotective Agent, Carboxylated ε-poly-L-lysine
Authors: Watanabe, Hitomi
Kohaya, Natsuki
Kamoshita, Maki
Fujiwara, Katsuyoshi
Matsumura, Kazuaki
Hyon, Suong-Hyu
Ito, Junya
Kashiwazaki, Naomi
Issue Date: 2013-12-23
Publisher: PLOS
Magazine name: PLoS One
Volume: 8
Number: 12
Start page: e83613
DOI: 10.1371/journal.pone.0083613
Abstract: In cryopreservation of mammalian germ cells, unfertilized oocytes are one of the most available stages because these cryopreserved oocytes can be used for assisted reproductive technologies, including in vitro fertilization (IVF) and intracytoplasmic sperm injection. However, it has been generally reported that the fertility and developmental ability of the oocytes are reduced by cryopreservation. Therefore further improvement will be required. Very recently, a new cryoprotective agent (CPA), called as carboxylated ε-poly-L-lysine (COOH-PLL), has been developed to reduce physical and physiological damage by cryopreservation in mammalian stem cells. However, it is unclear the effect of COOH-PLL on fertility and developmental ability of vitrified oocytes. In this study, we used COOH-PLL as a CPA with ethylene glycol (EG) for vitrification of mouse oocytes. Cumulus-oocyte complexes (COCs) were collected from ICR mice and then vitrified with Cryotop using different concentration of COOH-PLL and EG. A combined treatment with COOH-PLL and EG showed high survival rate (more than 90%) of vitrified-warmed COCs after in vitro fertilization. In addition, the fertility and developmental ability of COCs vitrified with E20P10 [EG 20% (v/v) and COOH-PLL 10% (w/v)] or E15P15 group (EG 15% and COOH-PLL 15%) were significantly higher than those with E10P20 (EG10% and COOH-PLL 20%) or P30 group (PLL30%). The vitrified COCs in E20P10 group developed to term at a high success rate (46.2%) and it was significantly higher than that in control (E30) group (34.8%). Our present study demonstrated for the first time that COOH-PLL is effective for vitrification of mouse oocytes.
Rights: Watanabe H, Kohaya N, Kamoshita M, Fujiwara K, Matsumura K, et al. (2013) Efficient Production of Live Offspring from Mouse Oocytes Vitrified with a Novel Cryoprotective Agent, Carboxylated e-poly-L-lysine. PLoS ONE 8(12): e83613. doi:10.1371/journal.pone.0083613 (c)2013 Watanabe et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
URI: http://hdl.handle.net/10119/12326
Material Type: publisher
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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