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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/12829

Title: 膜ダイナミクスを利用した動物実験代替法
Other Titles: Development of an alternative method to animal test for evaluation of skin-irritation
Authors: 高木, 昌宏
Authors(alternative): Takagi, Masahiro
Keywords: リポソーム
Issue Date: 3-Jun-2015
Abstract: 界面活性剤の皮膚刺激評価法に動物実験(ドレイズ試験)があるが、代替法の開発が望まれる。リポソームに界面活性剤を添加することで起きる、膜ダイナミクスを解析し、皮膚刺激性の関係を考察した。高刺激性界面活性剤ではリポソームが膜揺らぎを経て膜陥入した後に、膜孔を形成するダイナミクスを観察した。低刺激性界面活性剤では膜揺らぎを経て破裂するダイナミクスを観察した。刺激が起こるメカニズムについて分子レベルで考察し、ダイナミクスの背景、そして皮膚刺激の背景には、膜断面積の増減、フリップフロップが関係していると考え、分子レベルでのモデルを構築した。リポソームの膜ダイナミクスは、代替法の有力候補である。 : We studied about development of an alternative method of Draize test(Animal Test for Skin Irritation). Comparing the membrane dynamics of TritonX-100(Irritant) with that of Tween20 (Non-irritant), we considered that the degrees of irritation by surfactants could be predicted qualitatively. The dynamics induced by SLES was similar to that induced by TritonX-100. On the other hand, the dynamics induced by amino acid surfactants(FLDS-L, ACMT-L) were similar to the dynamics induced by Tween20. We observed the membrane invagination and pore formation for irritating surfactant. On the other hand, the large deformation could not be observed in the case of non-irritating surfactant. The deformation process was analyzed by the acquisition of the excess surface area. In order to acquire the excess surface area, the surfactant molecules should be included in the inner leaflet of bilayers to obtain the excess surface area. Based on this idea, we proposed a new model for skin-irritation.
Description: 研究種目:挑戦的萌芽研究
Language: jpn
URI: http://hdl.handle.net/10119/12829
Appears in Collections:2014年度 (FY 2014)

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