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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/12853

Title: Charge-induced phase separation in lipid membranes
Authors: Himeno, Hiroki
Shimokawa, Naofumi
Komura, Shigeyuki
Andelman, David
Hamada, Tsutomu
Takagi, Masahiro
Keywords: phase separation
lipid bilayers
charged lipid
Issue Date: 2014-08-26
Publisher: Royal Society of Chemistry
Magazine name: Soft Matter
Volume: 10
Start page: 7959
End page: 7967
DOI: 10.1039/C4SM01089B
Abstract: The phase separation in lipid bilayers that include negatively charged lipids is examined experimentally. We observed phase-separated structures and determined the membrane miscibility temperatures in several binary and ternary lipid mixtures of unsaturated neutral lipid, dioleoylphosphatidylcholine (DOPC), saturated neutral lipid, dipalmitoylphosphatidylcholine (DPPC), unsaturated charged lipid, dioleoylphosphatidylglycerol (DOPG^(-)), saturated charged lipid, dipalmitoylphosphatidylglycerol (DPPG^(-)), and cholesterol. In binary mixtures of saturated and unsaturated charged lipids, the combination of the charged head with the saturation of hydrocarbon tail is a dominant factor for the stability of membrane phase separation. DPPG^(-) enhances phase separation, while DOPG^(-) suppresses it. Furthermore, the addition of DPPG^(-) to a binary mixture of DPPC/cholesterol induces phase separation between DPPG-rich and cholesterol-rich phases. This indicates that cholesterol localization depends strongly on the electric charge on the hydrophilic head group rather than on the ordering of the hydrocarbon tails. Finally, when DPPG^(-) was added to a neutral ternary system of DOPC/DPPC/Cholesterol (a conventional model of membrane rafts), a three-phase coexistence was produced. We conclude by discussing some qualitative features of the phase behaviour in charged membranes using a free energy approach.
Rights: Copyright (C) 2014 Royal Society of Chemistry. Hiroki Himeno, Naofumi Shimokawa, Shigeyuki Komura, David Andelman, Tsutomu Hamada, Masahiro Takagi, Soft Matter, 10, 2014, 7959-7967. http://dx.doi.org/10.1039/C4SM01089B - Reproduced by permission of The Royal Society of Chemistry
URI: http://hdl.handle.net/10119/12853
Material Type: author
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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