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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/13696

Title: 気孔の数を調節するシグナルが細胞の外から内へ伝達される仕組み
Other Titles: Mechanism for regulating stomatal density by signaling from outside to inside of cells
Authors: 大木, 進野
Authors(alternative): Shinya, Ohki
Keywords: 気孔
Issue Date: 1-Jun-2016
Abstract: 気孔密度を調節するペプチドホルモンと受容体タンパク質の相互作用を原子分解能で解明することが本課題の目的である。本課題ではまず大腸菌と植物培養細胞を利用して受容体タンパク質の各種ドメイン、フラグメントを調製する系を確立した。その後、これらを構造解析や相互作用実験に使用するため試料条件を探索した。各種デタージェントの効果を検討した結果、有るフラグメントについては有望な条件を見いだすことができ、非標識体試料の2次元NMRスペクトルを測定することに成功した。本課題期間中には本格的な構造解析・機能解析に至らなかったが、今後の展開を大いに加速させる基礎的な成果が得られた。 : Purpose of this project is exploring the interaction between peptide hormones regulating stomatal density and their receptors at atomic resolution. First of all, we had established expression systems of some domains and fragments of the receptors using E. coli and/or BY-2. Next, we had extensively searched the adequate sample solution condition for analyzing the interaction. As the result of this screening for estimating the utility of various detergents, we fortunately found a certain condition for the experiments. We successfully measured a two-dimensional NMR spectrum of the unlabeled sample in this condition. Unfortunately, the screening to find the good sample condition took very long time, so that deeper structural analyses were not performed. However, the preliminary results obtained here seem to promote the research work in near future.
Description: 基盤研究(C)(一般)
Language: jpn
URI: http://hdl.handle.net/10119/13696
Appears in Collections:平成27年度 (FY 2015)

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