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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/13713

Title: Coupling between pore formation and phase separation in charged lipid membranes
Authors: Himeno, Hiroki
Ito, Hiroaki
Higuchi, Yuji
Hamada, Tsutomu
Shimokawa, Naofumi
Takagi, Masahiro
Issue Date: 2015-12
Publisher: American Physical Society
Magazine name: Physical Review E
Volume: 92
Number: 6
Start page: 062713-1
End page: 062713-12
DOI: 10.1103/PhysRevE.92.062713
Abstract: We investigated the effect of charge on the membrane morphology of giant unilamellar vesicles (GUVs) composed of various mixtures containing charged lipids. We observed the membrane morphologies by fluorescent and confocal laser microscopy in lipid mixtures consisting of a neutral unsaturated lipid [dioleoylphosphatidylcholine (DOPC)], a neutral saturated lipid [dipalmitoylphosphatidylcholine (DPPC)], a charged unsaturated lipid [dioleoylphosphatidylglycerol (DOPG (−) ) ], a charged saturated lipid [dipalmitoylphosphatidylglycerol (DPPG (−) ) ], and cholesterol (Chol). In binary mixtures of neutral DOPC-DPPC and charged DOPC−DPPG (−) , spherical vesicles were formed. On the other hand, pore formation was often observed with GUVs consisting of DOPG (−) and DPPC. In a DPPC-DPPG (−) -Chol ternary mixture, pore-formed vesicles were also frequently observed. The percentage of pore-formed vesicles increased with the DPPG (−) concentration. Moreover, when the head group charges of charged lipids were screened by the addition of salt, pore-formed vesicles were suppressed in both the binary and ternary charged lipid mixtures. We discuss the mechanisms of pore formation in charged lipid mixtures and the relationship between phase separation and the membrane morphology. Finally, we reproduce the results seen in experimental systems by using coarse-grained molecular dynamics simulations.
Rights: Hiroki Himeno, Hiroaki Ito, Yuji Higuchi, Tsutomu Hamada, Naofumi Shimokawa, Masahiro Takagi , Physical Review E, 92(6), 2015, 062713-1-062713-12. Copyright 2015 by the American Physical Society. http://dx.doi.org/10.1103/PhysRevE.92.062713
URI: http://hdl.handle.net/10119/13713
Material Type: publisher
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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