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JAIST Repository >
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FY 2016 >
Please use this identifier to cite or link to this item:
https://hdl.handle.net/10119/14323
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| Title: | 遺伝子修復治療を目指した化学的RNA Editing法の確立 |
| Other Titles: | Establishment of chemical RNA editing method for genetic code resoration therapy |
| Authors: | 塚原, 俊文 |
| Authors(alternative): | Tsukahara, Toshifumi |
| Keywords: | 疾患治療
点変異
RNA editing
脱アミノ化
蛍光タンパク質
遺伝コード修復
RFLP
恒常的発現細胞 |
| Issue Date: | 7-Jun-2017 |
| Abstract: | BFP-mRNAに対して脱アミノ化誘起用ODNを用いて光化学的な部位特異的脱アミノ化処理を行った。光化学的RNA editingは、RT-PCR-RFLPとin vitro翻訳したタンパク質の蛍光を確認した。また、光化学的RNA editingした当該mRNAの全長をシークエンスし、他の箇所に変異が生じていないことも確認した。効率的な脱アミノ化を誘起するODNデザインについても検討し、相補的配列長が14塩基で、ヘアピン長が9塩基のODNが12.4%と最も高い光化学的RNA editing効率を示した。この結果は光化学的RNA editing法が疾患治療法として適用可能であることを示唆している。：Photochemical site-specific deamination was performed toward BFP-mRNA, by using oligodeoxynucleotides,ODNs, for inducing deamination. The base substitution by artificial RNA editing was confirmed by RT-PCR-RFLP analysis and fluorescence of in-vitro-translated proteins. Moreover, the mRNA sample was sequenced after photochemical RNA editing treatments, and confirmed that there was no mutation occurred except the target-site. We also investigated the ODN design that could induce efficient deamination. The ODN with complementary sequence length of 14 bases and hairpin length of 9 bases showed the highest photochemical RNA editing efficiency at 12.4%. This result suggests that the photochemical RNA editing is applicable as a theraputic method for genetic diseases. |
| Description: | 基盤研究(B)（一般）
研究期間：2013～2016
課題番号：25290072
研究者番号：60207339
研究分野：分子生物学 |
| Language: | jpn |
| URI: | https://hdl.handle.net/10119/14323 |
| Appears in Collections: | 2016年度 (FY 2016)
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Files in This Item:
| File |
Description |
Size | Format |
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| 25290072seika.pdf | | 579Kb | Adobe PDF | View/Open |
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