|
|
JAIST Repository >
School of Materials Science >
Articles >
Journal Articles >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10119/15276
|
| Title: | High yield matrix-free ionization of biomolecules by pulse-heating ion source |
| Authors: | Luo, Xi
Phan, Tue Trong
Sugiyama, Kiyotaka
Takamura, Yuzuru |
| Keywords: | miniatuarized mass spectroscopy
soft ionization source
pulse-heating ionization source
biomolecules |
| Issue Date: | 2017-11-09 |
| Publisher: | Nature Publishing Group |
| Magazine name: | Scientific Reports |
| Volume: | 7 |
| Start page: | Article number: 15170 |
| DOI: | 10.1038/s41598-017-15259-y |
| Abstract: | Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry has been widely used for biomolecular analysis. However, with conventional MALDI, it is difficult to analyse low-molecular-weight compounds because of the interference of matrix ion signals. Here, we report a matrix-free on-chip pulse-heating desorption/ionization (PHDI) method for a wide range of biomolecules ranging from low molecular-weight substances such as glycine (75.7 Da) to large species such as α-lactalbumin (14.2 kDa). Compared with the conventional MALDI, the matrix-free PHDI method affords high yields of singly charged ions with very less fragmentation and background using only one-pulse without light (laser). We believe that this new technique for matrix-free biomolecules analysis would overcome the limitations of the conventional MALDI. |
| Rights: | Xi Luo, Phan-Trong Tue, Kiyotaka Sugiyama and Yuzuru Takamura, Scientific Reports, 7, 2017, Article number: 15170. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2017 |
| URI: | http://hdl.handle.net/10119/15276 |
| Material Type: | publisher |
| Appears in Collections: | c10-1. 雑誌掲載論文 (Journal Articles)
|
Files in This Item:
| File |
Description |
Size | Format |
|---|
| 23946.pdf | | 2870Kb | Adobe PDF | View/Open |
|
All items in DSpace are protected by copyright, with all rights reserved.
|
