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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/15363

Title: Electrochemical Immunoassay Using Open Circuit Potential Detection Labeled by Platinum Nanoparticles
Authors: Charoenkitamorn, Kanokwan
Phan, Tue Trong
Kawai, Keiko
Chailapakul, Orawon
Takamura, Yuzuru
Keywords: electrochemical immunosensor
Screen-printed carbon electrode
Gold nanoparticles
Open circuit potential
Issue Date: 2018-02-03
Publisher: MDPI Publishing
Magazine name: Sensors
Volume: 18
Number: 2
Start page: 444
End page: 445
DOI: 10.3390/s18020444
Abstract: In this work, a simple electrochemical immunoassay based on platinum nanoparticles (PtNPs) using open circuit potential (OCP) detection was developed. The detection of human chorionic gonadotropin hormone (hCG) as a model analyte, was demonstrated by direct electrical detection of PtNPs in hydrazine solution using OCP measurement without any application of either potential or current to the system. Disposable screen-printed carbon electrodes (SPCEs) were utilized for the development of our immunosensor, which required a sample volume as small as 2μL. After preparation of a sandwich-type immunosystem, hydrazine solution was dropped on the electrode’s surface, which was followed immediately by electrical detection using OCP. The change of the OCP signal originated from electrocatalytic oxidation of the hydrazine on PtNPs. Under the optimal conditions of a pH of 6.0 and a hydrazine concentration of 1 mM, a detection limit of 0.28 ng mL^<−1> and a linearity of 0–10 ng mL^<−1> were obtained. The PtNP-based OCP method is a simpler electrochemical detection procedure than those obtained from other electrochemical methods and has an acceptable sensitivity and reproducibility. The simplicity of the detection procedure and the cost-effectiveness of the disposable SPCE illustrate the attractive benefits of this sensor. Moreover, it could be applied to a simplified and miniaturized diagnostic system with minimal user manipulation.
Rights: Kanokwan Charoenkitamorn, Phan Trong Tue, Keiko Kawai, Orawon Chailapakul, and Yuzuru Takamura, Sensors, 18(2), 2018, 444-445. DOI:10.3390/s18020444 © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
URI: http://hdl.handle.net/10119/15363
Material Type: publisher
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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