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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/15418

Title: Thixotropic injectable hydrogel using a polyampholyte and nanosilicate prepared directly after cryopreservation
Authors: Jain, Minkle
Matsumura, Kazuaki
Keywords: thixotropy
injectable hydrogel
Issue Date: 2016-08-16
Publisher: Elsevier
Magazine name: Materials Science and Engineering: C
Volume: 69
Start page: 1273
End page: 1281
DOI: 10.1016/j.msec.2016.08.030
Abstract: Success of tissue engineering applications in regenerative medicine requires the preservation of tissue-engineered products at a low temperature. This can be successfully achieved by the use of cryoprotective agent (CPA). In this study, we formulated a unique injectable hydrogel for the purpose of cell delivery after cryopreservation by using polyampholyte CPA. The polyampholyte showed excellent post-thaw cell survival, and after thawing, the polymeric CPA did not have to be removed because of its low cytotoxicity. The polyampholyte could be transformed into a hydrogel by mixing with nanosilicates. Previously, nanosilicates were used to improve mechanical properties, but this is the first report of the use of a nanosilicate together with CPA to formulate hydrogels. Inclusion of the nanosilicate led to the formation of thixotropic hydrogels, which can be injected using fine needles. These gels with tunable mechanical properties can be injected into defect sites to form scaffolds for cell growth and tissue repair, and they do not require any separate seeding of cells before injection, thus eliminating the need for cell harvesting and cell maintenance. This is a distinct system in which cells can be cryopreserved until before usage; when required, the cells in the polyampholyte can be revived to their original state and the thixotropic hydrogel can be formed. The combination of thixotropy and cytocompatibility of the gels could enable a wide range of biomedical applications such as cell delivery and orthopedic repair.
Rights: Copyright (C)2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license (CC BY-NC-ND 4.0). [http://creativecommons.org/licenses/by-nc-nd/4.0/] NOTICE: This is the author's version of a work accepted for publication by Elsevier. Minkle Jain, Kazuaki Matsumura, Materials Science and Engineering: C, 69, 2016, 1273-1281, http://dx.doi.org/10.1016/j.msec.2016.08.030
URI: http://hdl.handle.net/10119/15418
Material Type: author
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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