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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/15878

Title: Comparative analysis of the cellular entry of polystyrene and gold nanoparticles using the freeze concentration method
Authors: Ahmed, Sana
Okuma, Koyo
Matsumura, Kazuaki
Keywords: freeze concentration
endocytic mechanism
Issue Date: 2018-04-30
Publisher: Royal Society of Chemistry
Magazine name: Biomaterials Science
Volume: 6
Start page: 1791
End page: 1799
DOI: 10.1039/C8BM00206A
Abstract: Despite advances in nanoparticle delivery, established physical approaches, such as electroporation and sonication, result in cell damage, limiting their practical applications. In this study, we proposed a unique freeze concentration-based technique and evaluated the efficacy of the method using two types of nanoparticles, citrate-capped gold nanoparticles and carboxylated polystyrene nanoparticles. We further compared the internalisation behaviour of particles of various sizes with and without freezing. Confocal microscopic images showed that the uptake efficacy for nanomaterials of 50 nm was greater than that of l00-nm particles. Polystyrene nanoparticles of 50 nm had more favourable adsorption and internalisation behaviours compared to those of gold nanoparticles after freeze concentration. We also examined the possible endocytic pathways involved in the uptake of gold and polystyrene nanoparticles, and found that the route differed between unfrozen and frozen conditions. Overall, we determined the influence of the freeze concentration strategy on both nanomaterial internalisation and the endocytic uptake pathway. Our findings provide a mechanistic understanding of the internalisation of nanoparticles using a freezing approach and thereby contribute to further developments in nanotherapeutic applications.
Rights: Copyright (C) 2018 Royal Society of Chemistry. Sana Ahmed, Koyo Okuma and Kazuaki Matsumura, Biomaterials Science, 6, 2018, 1791-1799. http://dx.doi.org/10.1039/C8BM00206A - Reproduced by permission of The Royal Society of Chemistry
URI: http://hdl.handle.net/10119/15878
Material Type: author
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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