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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/15992

Title: Comparative Study of Protein Aggregation Arrest by Zwitterionic Polysulfobetaines: Using Contrasting Raft Agents
Authors: Sharma, Neha
Rajan, Robin
Makhaik, Sparsh
Matsumura, Kazuaki
Issue Date: 2019-07-15
Publisher: American Chemical Society
Magazine name: ACS Omega
Volume: 4
Number: 7
Start page: 12186
End page: 12193
DOI: 10.1021/acsomega.9b01409
Abstract: Protein aggregation has caused limitations in the study and development of protein-based biopharmaceuticals. We prepared different polysulfobetaine (poly-SPB) polymers via reversible addition fragmentation chain transfer (RAFT) polymerization. These polymers exhibited high efficiency in modulation of protein aggregation. We synthesized polysulfobetaines using two different RAFT agents, and analyzed the aggregation profile of lysozyme and insulin. In poly-SPBs, existence of a hydrophobic RAFT agent resulted in visible enhancement of the residual enzymatic activity of lysozyme, whereas it remained unaffected by the hydrophilic RAFT agent. In addition, these polymers resulted in significant suppression in the aggregation of insulin. Increase in the molecular weight of the polymer caused higher efficiency to perpetuate enzymatic activity of lysozyme upon thermal denaturation. The polymers arrested the formation of amyloid like fibrils of lysozyme and insulin, thus indicating their potential to inhibit aggregation. The results unambiguously demonstrate the importance of polysulfobetaine moiety and hydrophobicity in protein aggregation inhibition. This study gives insight into the protein aggregation inhibition by zwitterionic polymers, which have a potential to be developed as aggregation inhibitors in the future.
Rights: This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. Copyright (c) 2019 American Chemical Society. Neha Sharma, Robin Rajan, Sparsh Makhaik, and Kazuaki Matsumura, ACS Omega, 4(7), 2019, 12186-12193. DOI:10.1021/acsomega.9b01409
URI: http://hdl.handle.net/10119/15992
Material Type: publisher
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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