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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/16966

Title: 新規凍結保護物質の合成とその応用
Authors: 松村, 和明
Issue Date: 2016
Publisher: 低温生物工学会
Magazine name: 低温生物工学会誌 = Cryobiology and cryotechnology
Volume: 62
Number: 1
Start page: 11
End page: 15
DOI: 10.20585/cryobolcryotechnol.62.1_11
Abstract: We developed novel cryoprotective agents from carboxylated ε-poly-L-lysines (COOH-PLLs) for cell cryopreservation as alternatives to dimethyl sulfoxide (DMSO). The polymeric agents showed excellent cell viability profile for most cell types, including human mesenchymal stem cells. We investigated the molecular mechanism of cryopreservation of the polymers by using solid-state nuclear magnetic resonance (NMR). The NMR data suggested that DMSO and COOH-PLLs differently affect the states and mobility of water and solutes in ice. The polyampholytes cryoprotectants showed highly ice recrystallization inhibition properties and we are developing vitrification solution for stem cells and tissue-engineered constructs cryopreservation using the ability. Through in situ crosslinking via click chemistry, the polyampholyte cryoprotectant could become a cell scaffold hydrogel with cryoprotective properties for tissue engineering application. We also discovered novel protein delivery method by using freeze concentration mechanism by using the polyampholyte as a cryoprotectant and protein nanocarrier.
Rights: 本著作物は低温生物工学会の許可のもとに掲載するものです。This material is posted here with permission of the Japanese Society for Cryobiology and Cryotechnology. Copyright (C) 2016 低温生物工学会. 松村 和明, 低温生物工学会誌 = Cryobiology and cryotechnology, 62(1), 2016, pp.11-15. http://dx.doi.org/10.20585/cryobolcryotechnol.62.1_11
URI: http://hdl.handle.net/10119/16966
Material Type: publisher
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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