|
JAIST Repository >
School of Materials Science >
Articles >
Journal Articles >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10119/16967
|
| Title: | Novel Concentration-Based Freezing Method for Efficient Protein Delivery |
| Authors: | AHMED, Sana
MATSUMURA, Kazuaki |
| Issue Date: | 2016 |
| Publisher: | 低温生物工学会 |
| Magazine name: | 低温生物工学会誌 = Cryobiology and cryotechnology |
| Volume: | 62 |
| Number: | 2 |
| Start page: | 143 |
| End page: | 147 |
| DOI: | 10.20585/cryobolcryotechnol.62.2_143 |
| Abstract: | We developed a novel, well-controlled slow-freezing concentration-based strategy for effective protein delivery using polyampholyte NPs in the absence of a cryoprotectant. Here, we demonstrate the feasibility of this freeze concentration method, which is exceptionally favorable for cytoplasmic protein delivery, and focus on a simple slow freeze-thaw method using slow freezing program. We used confocal laser scanning microscopy to investigate the adsorption and internalization of lysozyme proteins at variable freezing temperatures. Quantification of fluorescence intensity revealed that proteins were more easily internalized by gradually decreasing the temperature. We believe that application of this concentration-based freezing technique could be extended to gene therapy and cancer immunotherapy. |
| Rights: | 本著作物は低温生物工学会の許可のもとに掲載するものです。This material is posted here with permission of the Japanese Society for Cryobiology and Cryotechnology. Copyright (C) 2016 低温生物工学会. Sana AHMED, Kazuaki MATSUMURA, 低温生物工学会誌 = Cryobiology and cryotechnology, 62(2), 2016, pp.143-147. http://dx.doi.org/10.20585/cryobolcryotechnol.62.2_143 |
| URI: | http://hdl.handle.net/10119/16967 |
| Material Type: | publisher |
| Appears in Collections: | c10-1. 雑誌掲載論文 (Journal Articles)
|
Files in This Item:
| File |
Description |
Size | Format |
|---|
| 3364.pdf | | 718Kb | Adobe PDF | View/Open |
|
All items in DSpace are protected by copyright, with all rights reserved.
|
