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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/16967

Title: Novel Concentration-Based Freezing Method for Efficient Protein Delivery
Authors: AHMED, Sana
MATSUMURA, Kazuaki
Issue Date: 2016
Publisher: 低温生物工学会
Magazine name: 低温生物工学会誌 = Cryobiology and cryotechnology
Volume: 62
Number: 2
Start page: 143
End page: 147
DOI: 10.20585/cryobolcryotechnol.62.2_143
Abstract: We developed a novel, well-controlled slow-freezing concentration-based strategy for effective protein delivery using polyampholyte NPs in the absence of a cryoprotectant. Here, we demonstrate the feasibility of this freeze concentration method, which is exceptionally favorable for cytoplasmic protein delivery, and focus on a simple slow freeze-thaw method using slow freezing program. We used confocal laser scanning microscopy to investigate the adsorption and internalization of lysozyme proteins at variable freezing temperatures. Quantification of fluorescence intensity revealed that proteins were more easily internalized by gradually decreasing the temperature. We believe that application of this concentration-based freezing technique could be extended to gene therapy and cancer immunotherapy.
Rights: 本著作物は低温生物工学会の許可のもとに掲載するものです。This material is posted here with permission of the Japanese Society for Cryobiology and Cryotechnology. Copyright (C) 2016 低温生物工学会. Sana AHMED, Kazuaki MATSUMURA, 低温生物工学会誌 = Cryobiology and cryotechnology, 62(2), 2016, pp.143-147. http://dx.doi.org/10.20585/cryobolcryotechnol.62.2_143
URI: http://hdl.handle.net/10119/16967
Material Type: publisher
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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