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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/16968

Title: Role of Cryoprotectants in Freeze Concentration Technology for Gene Delivery Process
Authors: AHMED, Sana
MIYAWAKI, Osato
MATSUMURA, Kazuaki
Issue Date: 2017
Publisher: 低温生物工学会
Magazine name: 低温生物工学会誌 = Cryobiology and cryotechnology
Volume: 63
Number: 2
Start page: 147
End page: 154
DOI: 10.20585/cryobolcryotechnol.63.2_147
Abstract: There is an urgent need to develop highly effective techniques for successful and efficient gene delivery. In this study, we demonstrated the use of cryoprotectants in the newly developed freeze concentration technique in the gene delivery system. We found that at extremely low temperatures, gene transfection was effectively enhanced. Additionally, by comparing the effects of the commercially available cryoprotectant DMSO against a synthesized polyampholyte cryoprotectant, we were able to establish that the latter was more effective at increasing the rate of successful gene transfection. This particular feature could be attributed to the higher freeze concentration factor of polyampholytes in comparison with DMSO, under frozen conditions. Therefore, the study demonstrated the unique efficacy of polyampholytes as a cryoprotectant using the freeze concentration method for efficient in vitro gene delivery.
Rights: 本著作物は低温生物工学会の許可のもとに掲載するものです。This material is posted here with permission of the Japanese Society for Cryobiology and Cryotechnology. Copyright (C) 2017 低温生物工学会. Sana AHMED, Osato MIYAWAKI, Kazuaki MATSUMURA, 低温生物工学会誌 = Cryobiology and cryotechnology, 63(2), 2017, pp.147-154. http://dx.doi.org/10.20585/cryobolcryotechnol.63.2_147
URI: http://hdl.handle.net/10119/16968
Material Type: publisher
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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