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このアイテムの引用には次の識別子を使用してください:
http://hdl.handle.net/10119/17042
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| タイトル: | A Freeze‐Concentration and Polyampholyte‐Modified Liposome‐Based Antigen‐Delivery System for Effective Immunotherapy |
| 著者: | Ahmed, Sana
Fujita, Satoshi
Matsumura, Kazuaki |
| キーワード: | freeze-concentration
immunotherapy
polyampholyte
liposomes |
| 発行日: | 2017-05-11 |
| 出版者: | WILEY‐VCH |
| 誌名: | Advanced Healthcare Materials |
| 巻: | 6 |
| 号: | 14 |
| 開始ページ: | 1700207 |
| DOI: | 10.1002/adhm.201700207 |
| 抄録: | Immunotherapy is an exciting new approach in cancer treatment. Here, we describe the development of a novel freeze-concentration method that could be applicable in immunotherapy. The method involves freezing cells in the presence of pH-sensitive, polyampholyte-modified liposomes with encapsulated ovalbumin (OVA) as the antigen. In RAW 264.7 cells, compared to the non-frozen condition, freeze-concentration of polyampholyte-modified liposomes encapsulating OVA resulted in efficient OVA uptake and also allowed for its delivery to the cytosol. Efficient delivery of OVA to the cytosol was shown to be partly due to the pH-dependence of the polyampholyte-modified liposomes. Cytosolic OVA delivery also resulted in significant up-regulation of the major histocompatibility complex class I pathway through a process known as cross-stimulation, as well as an increase in the release of IL-1β, IL-6, and TNF-α. Our results demonstrate that the combination of a simple freeze-concentration method and polyampholyte-modified liposomes might be useful in future immunotherapy applications. |
| Rights: | (c) 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Sana Ahmed, Satoshi Fujita, Kazuaki Matsumura, Advanced Healthcare Materials, 6(14), 2017, 1700207, which has been published in final form at https://doi.org/10.1002/adhm.201700207. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
| URI: | http://hdl.handle.net/10119/17042 |
| 資料タイプ: | author |
| 出現コレクション: | c10-1. 雑誌掲載論文 (Journal Articles)
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このアイテムのファイル:
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記述 |
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| 3413.pdf | | 2138Kb | Adobe PDF | 見る/開く |
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