|
|
JAIST Repository >
c. マテリアルサイエンス研究科・マテリアルサイエンス系 >
c10. 学術雑誌論文等 >
c10-1. 雑誌掲載論文 >
このアイテムの引用には次の識別子を使用してください:
http://hdl.handle.net/10119/17044
|
| タイトル: | Cytosolic delivery of quantum dots mediated by freezing and hydrophobic polyampholytes in RAW 264.7 cells |
| 著者: | Ahmed, Sana
Nakaji-Hirabayashi, Tadashi
Rajan, Robin
Zhao, Dandan
Matsumura, Kazuaki |
| 発行日: | 2019-10-29 |
| 出版者: | Royal Society of Chemistry |
| 誌名: | Journal of Materials Chemistry B |
| 巻: | 7 |
| 号: | 46 |
| 開始ページ: | 7387 |
| 終了ページ: | 7395 |
| DOI: | 10.1039/C9TB01184F |
| 抄録: | Quantum dots (QDs) can be delivered efficiently inside macrophages using a freeze-concentration approach. In this study, we introduced a new, facile, high concentration-based freezing technology of low toxicity. We also developed QD-conjugated new hydrophobic polyampholytes using poly-L-lysine (PLL), a naturally derived polymer, which showed sustained biocompatibility, stability over one week, and enhanced intracellular delivery. When freeze-concentration was applied, the QD-encapsulated hydrophobic polyampholytes showed a higher tendency to adsorb onto the cell membrane than the non-frozen molecules. Interestingly, we observed that the efficacy of adsorption of QDs on RAW 264.7 macrophages was higher than that on fibroblasts. Furthermore, the intracellular delivery of QDs using hydrophobic polyampholytes was higher than those of PLL and QDs. In vitro studies revealed the efficient endosomal escape of QDs in the presence of hydrophobic polyampholytes and freeze-concentration. Collectively, these observations indicated that the promising combination of freeze-concentration and hydrophobic polyampholytes may act as an effective and versatile strategy for the intracellular delivery of QDs, which can be used for biological diagnosis and therapeutic applications. |
| Rights: | Copyright (C) 2019 Royal Society of Chemistry. Sana Ahmed, Tadashi Nakaji-Hirabayashi, Robin Rajan, Dandan Zhao and Kazuaki Matsumura, Journal of Materials Chemistry B, 7(46), 2019, 7387-7395. http://dx.doi.org/10.1039/C9TB01184F - Reproduced by permission of The Royal Society of Chemistry |
| URI: | http://hdl.handle.net/10119/17044 |
| 資料タイプ: | author |
| 出現コレクション: | c10-1. 雑誌掲載論文 (Journal Articles)
|
このアイテムのファイル:
| ファイル |
記述 |
サイズ | 形式 |
|---|
| 3417.pdf | | 1568Kb | Adobe PDF | 見る/開く |
|
当システムに保管されているアイテムはすべて著作権により保護されています。
|
