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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/17044

Title: Cytosolic delivery of quantum dots mediated by freezing and hydrophobic polyampholytes in RAW 264.7 cells
Authors: Ahmed, Sana
Nakaji-Hirabayashi, Tadashi
Rajan, Robin
Zhao, Dandan
Matsumura, Kazuaki
Issue Date: 2019-10-29
Publisher: Royal Society of Chemistry
Magazine name: Journal of Materials Chemistry B
Volume: 7
Number: 46
Start page: 7387
End page: 7395
DOI: 10.1039/C9TB01184F
Abstract: Quantum dots (QDs) can be delivered efficiently inside macrophages using a freeze-concentration approach. In this study, we introduced a new, facile, high concentration-based freezing technology of low toxicity. We also developed QD-conjugated new hydrophobic polyampholytes using poly-L-lysine (PLL), a naturally derived polymer, which showed sustained biocompatibility, stability over one week, and enhanced intracellular delivery. When freeze-concentration was applied, the QD-encapsulated hydrophobic polyampholytes showed a higher tendency to adsorb onto the cell membrane than the non-frozen molecules. Interestingly, we observed that the efficacy of adsorption of QDs on RAW 264.7 macrophages was higher than that on fibroblasts. Furthermore, the intracellular delivery of QDs using hydrophobic polyampholytes was higher than those of PLL and QDs. In vitro studies revealed the efficient endosomal escape of QDs in the presence of hydrophobic polyampholytes and freeze-concentration. Collectively, these observations indicated that the promising combination of freeze-concentration and hydrophobic polyampholytes may act as an effective and versatile strategy for the intracellular delivery of QDs, which can be used for biological diagnosis and therapeutic applications.
Rights: Copyright (C) 2019 Royal Society of Chemistry. Sana Ahmed, Tadashi Nakaji-Hirabayashi, Robin Rajan, Dandan Zhao and Kazuaki Matsumura, Journal of Materials Chemistry B, 7(46), 2019, 7387-7395. http://dx.doi.org/10.1039/C9TB01184F - Reproduced by permission of The Royal Society of Chemistry
URI: http://hdl.handle.net/10119/17044
Material Type: author
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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