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このアイテムの引用には次の識別子を使用してください:
http://hdl.handle.net/10119/17052
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| タイトル: | Molecular mechanisms of cell cryopreservation with polyampholytes studied by solid-state NMR |
| 著者: | Matsumura, Kazuaki
Hayashi, Fumiaki
Nagashima, Toshio
Rajan, Robin
Hyon, Suong-Hyu |
| 発行日: | 2021-02-09 |
| 出版者: | Springer Nature |
| 誌名: | Communications Materials |
| 巻: | 2 |
| 開始ページ: | 15 |
| DOI: | 10.1038/s43246-021-00118-1 |
| 抄録: | Polyampholytes are emerging macromolecular membrane non-penetrating cryoprotectants; however, the mechanism behind their cryopreservation remains unclear. Here, we investigated the mechanism using solid-state NMR spectroscopy. The polymer-chain dynamics and the water and ion mobilities in the presence of various membrane penetrating and non-penetrating cryoprotectants were monitored at low temperatures to mimic cryopreservation conditions. NMR experiments revealed that the water, Sodium-ion, and polymer-chain signals in a carboxylated poly-ʟ-lysine (COOH-PLL) solution broadened upon cooling, indicating increasingly restricted mobility and increased solution viscosity. Moreover, strong intermolecular interactions facilitated the COOH-PLL glass transition, trapping water and salt in the gaps of the reversible matrix, preventing intracellular ice formation and osmotic shock during freezing; this reduced cell stress is responsible for cryoprotection. This simple NMR technique enabled the correlation of the cryoprotective properties of polymers that operate through mechanisms different from those of current cryoprotectants, and will facilitate the future molecular design of cryoprotectants. |
| Rights: | (c) The Author(s) 2021. Kazuaki Matsumura, Fumiaki Hayashi, Toshio Nagashima, Robin Rajan and Suong-Hyu Hyon, Communications Materials, 2, 2021, Article number: 15. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| URI: | http://hdl.handle.net/10119/17052 |
| 資料タイプ: | publisher |
| 出現コレクション: | c10-1. 雑誌掲載論文 (Journal Articles)
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