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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/17577

Title: Molecular Design of Polyampholytes for Vitrification-Induced Preservation of Three-Dimensional Cell Constructs without Using Liquid Nitrogen
Authors: Matsumura, Kazuaki
Hatakeyama, Sho
Naka, Toshiaki
Ueda, Hiroshi
Rajan, Robin
Tanaka, Daisuke
Hyon, Suong-Hyu
Issue Date: 2020-07-13
Publisher: American Chemical Society
Magazine name: Biomacromolecules
Volume: 21
Number: 8
Start page: 3017
End page: 3025
DOI: 10.1021/acs.biomac.0c00293
Abstract: Current slow-freezing methods are too inefficient for cryopreservation of three-dimensional (3D) tissue constructs. Additionally, conventional vitrification methods use liquid nitrogen, which is inconvenient and increases the chance of cross-contamination. Herein, we have developed polyampholytes with various degrees of hydrophobicity and showed that they could successfully vitrify cell constructs including spheroids and cell monolayers without using liquid nitrogen. The polyampholytes prevented ice crystallization during both cooling and warming, demonstrating their potential to prevent freezing-induced damage. Monolayers and spheroids vitrified in the presence of polyampholytes yielded high viabilities post-thawing with monolayers vitrified with PLL-DMGA exhibiting more than 90% viability. Moreover, spheroids vitrified in the presence of polyampholytes retained their fusibilities, thus revealing the propensity of these polyampholytes to stabilize 3D cell constructs. This study is expected to open new avenues for the development of off-the-shelf tissue engineering constructs that can be prepared and preserved until needed.
Rights: This is an open access article published under an ACS AuthorChoice License (https://acsopenscience.org/open-access/licensing-options/), which permits copying and redistribution of the article or any adaptations for non-commercial purposes. Kazuaki Matsumura, Sho Hatakeyama, Toshiaki Naka, Hiroshi Ueda, Robin Rajan, Daisuke Tanaka, and Suong-Hyu Hyon, Biomacromolecules, 2020, 21(8), pp.3017-3025. DOI:10.1021/acs.biomac.0c00293
URI: http://hdl.handle.net/10119/17577
Material Type: publisher
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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