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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/18081

Title: Gene expression analysis of human induced pluripotent stem cells cryopreserved by vitrification using StemCell Keep
Authors: Ota, Akemi
Hyon, Suong-Hyu
Sumi, Shoichiro
Matsumura, Kazuaki
Keywords: Human induced pluripotent stem cell
Cryopreservation
Vitrification
DNA microarray
Gene enrichment analysis
Issue Date: 2021-11-15
Publisher: Elsevier
Magazine name: Biochemistry and Biophysics Reports
Volume: 28
Start page: 101172
DOI: 10.1016/j.bbrep.2021.101172
Abstract: In recent years, regenerative medicine research using human somatic and induced pluripotent stem cells has advanced considerably, promoting clinical applications. However, it is essential that these cells are cryopreserved safely and effectively. Most cryopreservation solution agents contain dimethyl sulfoxide (DMSO), which exhibits strong toxicity and can potentially promote cell differentiation. Hence, it is important to explore substitutes for DMSO in cryoprotectant solutions. One such alternative is StemCell Keep (SCK), a DMSO-free solution that has been reported to effectively cryopreserve human induced pluripotent stem cells (hiPS cells). To clarify the effect of cryopreservation agents on cells, DNA microarray analysis is useful, as it can identify a large number of gene expression differences in cryopreserved cells, as well as functional increases in gene groups. In this study, we performed gene expression analysis of SCK-cryopreserved hiPS cells using a DNA microarray gene chip. The hiPS cells vitrified with SCK or DMSO-based vitrification solutions were thawed and cultured on Matrigel under feeder-free conditions, and RNA was extracted for DNA microarray analysis. Genes obtained from DNA microarray data were classified by the keywords of Gene Ontology Biological Process Term, and their relationships were analyzed using DAVID or the GeneMANIA database. SCK-cryopreserved hiPS cells expressed several anti-apoptotic genes, as well as genes related to cell adhesion or proliferation at levels that were nearly equivalent to those of non-frozen hiPS cells. Gene enrichment analysis with selected genes of SCK-cryopreserved hiPS cells whose expression differences were superior to those of DAP-cryopreserved showed strong interactions of negative regulation of apoptotic process, cell adhesion and positive regulation of cell proliferation in DAVID analysis. We demonstrated that SCK successfully maintained the key functions of hiPS cells, including anti-apoptosis, cell adhesion, and cell proliferation, during cryopreservation.
Rights: (c)2021 The Author(s). Published by ElsevierB.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). Akemi Ota, Suong-Hyu Hyon, Shoichiro Sumi, Kazuaki Matsumura, Biochemistry and Biophysics Reports,2021,28,101172. DOI:10.1016/j.bbrep.2021.101172
URI: http://hdl.handle.net/10119/18081
Material Type: publisher
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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