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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/4630

Title: Composition Analysis of Polar Lipids in Halobacteria with Mass Spectrometry
Authors: NOGUCHI, Yuuki
HAYASHI, Akio
TSUJIMOTO, Kazuo
MIYABAYASHI, Keiko
MIZUKAMI, Taku
NAITO, Yasuhide
OHASHI, Mamoru
Keywords: Halobacteria
Phospholipid
Glycolipid sulfate
ESI-MS
FAB-MS
Negative ion
Chemotaxonomy
MS/MS analysis
Issue Date: 2004
Publisher: 日本質量分析学会
Magazine name: Journal of the Mass Spectrometry Society of Japan
Volume: 52
Number: 6
Start page: 307
End page: 316
Abstract: The structures of the polar lipids in halobacteria including their mutants were determined with ESI-MS and FAB-MS. The composition-analyses with ESI-MS gave the results fairly close to these with TLC analyses. The percentages of the composite polar lipids by the mass spectrometric analyses of Halobacterium salinarum S-9 were 20, 77, and 5% for glycolipid sulfate (S-TGD), methyl ester of phosphatidyl glycerophosphate (PGP-OMe), and phosphatidylglycerol (PG), respectively. The MS/MS analysis in the FAB-MS provided an analyzing tool for the structural elucidation of the functional groups in the lipids. Newly proposed methylation in the phosphate was investigated with stable isotope experiments. We found that the Australian halobacteria, Halorubrum sp. aus-1, mainly consist of an isolated glycolipid sulfate with two sugar parts of glucopyranoses. In relationship with the lipid-composition analysis, the chemotaxonomy of halobacteria was discussed. The methylation of the lipids was proved to be essentially independent of that in the photosensory protein of halobacteria on the basis of the wide distribution of the methylated lipids.
Rights: Yuuki NOGUCHI, Akio HAYASHI, Kazuo TSUJIMOTO, Keiko MIYABAYASHI, Taku MIZUKAMI, Yasuhide NAITO and Mamoru OHASHI (2004) Composition Analysis of Polar Lipids in Halobacteria with Mass Spectrometry . J. Mass Spectrom. Soc. Jpn. 52, 307-316. ここに掲載した著作物の利用に関する注意:本著作物の著作権は日本質量分析学会に帰属します。本著作物は著作権者である日本質量分析学会の許可のもとに掲載するものです。
URI: http://hdl.handle.net/10119/4630
Material Type: publisher
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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