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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/4933

Title: Preparation and characterization of biodegradable nanoparticles based on poly(γ-glutamic acid) with L-phenylalanine as a protein carrier
Authors: Akagi, Takami
Kaneko, Tatsuo
Kida, Toshiyuki
Akashi, Mitsuru
Keywords: Poly (γ-glutamic acid)
Biodegradation
Nanoparticles
Amphiphilic
Encapsulation
Issue Date: 2005-11-28
Publisher: Elsevier
Magazine name: Journal of Controlled Release
Volume: 108
Number: 2-3
Start page: 226
End page: 236
DOI: 10.1016/j.jconrel.2005.08.003
Abstract: The objective of the present study was to prepare nanoparticles composed of poly (γ-glutamic acid) (γ-PGA) and L-phenylalanine ethylester (L-PAE) in order to evaluate the possibility of using these nanoparticles as protein carriers. Novel amphiphilic graft copolymers composed of γ-PGA as the hydrophilic backbone and L-PAE as the hydrophobic segment were successfully synthesized by grafting L-PAE to γ-PGA using water-soluble carbodiimide (WSC). Due to their amphiphilic properties, the γ-PGA-graft-L-PAE copolymers were able to form nanoparticles. The size of the γ-PGA nanoparticles was measured by photon correlation spectroscopy (PCS), and showed a narrow monodispersed size distribution with a mean diameter ranging from 150 to 200 nm. The solvents selected to prepare the γ-PGA nanoparticles by a precipitation and dialysis method affected the particle size distribution. To evaluate the feasibility of vehicles for these proteins, we prepared protein-loaded γ-PGA nanoparticles by surface immobilization and encapsulation methods. Ovalbumin (OVA) was used as a model protein, and was immobilized onto the γ-PGA nanoparticles or encapsulated into the inner core of these nanoparticles. Moreover, these OVA-encapsulated γ-PGA nanoparticles could be preserved by freeze-drying process. The results of cytotoxicity tests showed that the γ-PGA and γ-PGA nanoparticles did not cause any relevant cell damage. It is expected that biodegradable γ-PGA nanoparticles can immobilize proteins, peptides, plasmid DNA and drugs onto their surfaces and/or into the nanoparticles. These nanoparticles are potentially useful in pharmaceutical and biomedical applications.
Rights: NOTICE: This is the author's version of a work accepted for publication by Elsevier. Takami Akagi, Tatsuo Kaneko, Toshiyuki Kida, and Mitsuru Akashi, Journal of Controlled Release, 108(2-3), 2005, 226-236, http://dx.doi.org/10.1016/j.jconrel.2005.08.003
URI: http://hdl.handle.net/10119/4933
Material Type: author
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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