|
JAIST Repository >
c. マテリアルサイエンス研究科・マテリアルサイエンス系 >
c10. 学術雑誌論文等 >
c10-1. 雑誌掲載論文 >
このアイテムの引用には次の識別子を使用してください:
http://hdl.handle.net/10119/4945
|
| タイトル: | Peptides binding to a Gb3 mimic selected from a phage library |
| 著者: | Miura, Yoshiko
Sasao, Yuuki
Kamihira, Masamichi
Sakaki, Akio
Iijima, Shinji
Kobayashi, Kazukiyo |
| キーワード: | phage display peptide library
protein-oligosaccharide interaction
Gb3
Shiga toxin
quartz crystal microbalance |
| 発行日: | 2004-08-04 |
| 出版者: | Elsevier |
| 誌名: | Biochimica et Biophysica Acta (BBA) : General Subjects |
| 巻: | 1673 |
| 号: | 3 |
| 開始ページ: | 131 |
| 終了ページ: | 138 |
| DOI: | 10.1016/j.bbagen.2004.04.009 |
| 抄録: | Peptides binding to a Gb3 mimic were selected from 12mer peptide library. The self-assembled monolayer (SAM) of a Gb3 mimic was formed on the gold surface, and biopanning was carried out with the phage display peptide library. After 3 rounds of biopanning, four individual sequences were obtained from 10 phage clones, and the selected peptides having the specific 7mer sequence (FHENWPS) showed affinities to the Gb3 mimic as strong as to RCA_<120>. Molecular dynamics calculations suggested that the peptides bound to the Gb3 mimic by hydrophobic interaction and hydrogen bonding formation, and the cooperative interactions played an important role in the recognition. The Stx-1 binding was inhibited by the peptides. |
| Rights: | NOTICE: This is the author's version of a work accepted for publication by Elsevier. Yoshiko Miura, Yuuki Sasao, Masamichi Kamihira, Akio Sakaki, Shinji Iijima and Kazukiyo Kobayashi, Biochimica et Biophysica Acta (BBA) : General Subjects, 1673(3), 2004, 131-138, http://dx.doi.org/10.1016/j.bbagen.2004.04.009 |
| URI: | http://hdl.handle.net/10119/4945 |
| 資料タイプ: | author |
| 出現コレクション: | c10-1. 雑誌掲載論文 (Journal Articles)
|
このアイテムのファイル:
| ファイル |
記述 |
サイズ | 形式 |
|---|
| C4685.pdf | | 399Kb | Adobe PDF | 見る/開く |
|
当システムに保管されているアイテムはすべて著作権により保護されています。
|
