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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/8548

Title: Construction and high cytoplasmic expression of a tumoricidal single-chain antibody against hepatocellular carcinoma
Authors: Sandee, Duanpen
Tungpradabkul, Sumalee
Tsukio, Manae
Imanaka, Tadayuki
Takagi, Masahiro
Keywords: Single-chain Fv (scFv)
Hepatocellular carcinoma (HCC)
Bacterial expression
Inclusion body
Issue Date: 2002-09-12
Publisher: BioMed Central Ltd.
Magazine name: BMC Biotechnology
Volume: 2
Number: article no.16
DOI: 10.1186/1472-6750-2-16
Abstract: Background: Hep27 monoclonal (Hep27 Mab) is an antibody against hepatocellular carcinoma. Hep27 Mab itself can inhibit the growth of a hepatocellular carcinoma cell line (HCC-S102). We attempted to produce a single-chain fragment (scFv), a small fragment containing an antigen-binding site of Hep27 Mab, by using DNA-recombinant techniques. Results: The sequences encoding the variable regions of heavy (V_H) and light (V_L) chains of a murine Hep27 Mab were linked together by a linker peptide (Gly4Ser)_3 and tagged with a hexahistidine at the C-terminal; the resultant DNA construct was expressed in E. coli as an insoluble protein. The denatured scFv was refolded and purified by immobilized metal ion affinity chromatography (12 mg/l with a molecular weight of 27 kDa). Hep27scFv exhibited a tumoricidal activity against the HCC-S102 cell as its parental antibody (Hep27 Mab). Conclusion: This scFv may be a potential candidate for a targeting agent in HCC immunodiagnosis or immunotherapy.
Rights: BMC Biotechnology 2002, 2:16 This article is available from: http://www.biomedcentral.com/1472-6750/2/16 c 2002 Sandee et al; licensee BioMed Central Ltd. This article is published in Open Access: verbatim copying and redistribution of this article are permitted in all media for any non-commercial purpose, provided this notice is preserved along with the article's original URL.
URI: http://hdl.handle.net/10119/8548
Material Type: publisher
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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