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https://hdl.handle.net/10119/8548
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| Title: | Construction and high cytoplasmic expression of a tumoricidal single-chain antibody against hepatocellular carcinoma |
| Authors: | Sandee, Duanpen
Tungpradabkul, Sumalee
Tsukio, Manae
Imanaka, Tadayuki
Takagi, Masahiro |
| Keywords: | Single-chain Fv (scFv)
Hepatocellular carcinoma (HCC)
Bacterial expression
Inclusion body |
| Issue Date: | 2002-09-12 |
| Publisher: | BioMed Central Ltd. |
| Magazine name: | BMC Biotechnology |
| Volume: | 2 |
| Number: | article no.16 |
| DOI: | 10.1186/1472-6750-2-16 |
| Abstract: | Background: Hep27 monoclonal (Hep27 Mab) is an antibody against hepatocellular carcinoma. Hep27 Mab itself can inhibit the growth of a hepatocellular carcinoma cell line (HCC-S102). We attempted to produce a single-chain fragment (scFv), a small fragment containing an antigen-binding site of Hep27 Mab, by using DNA-recombinant techniques. Results: The sequences encoding the variable regions of heavy (V_H) and light (V_L) chains of a murine Hep27 Mab were linked together by a linker peptide (Gly4Ser)_3 and tagged with a hexahistidine at the C-terminal; the resultant DNA construct was expressed in E. coli as an insoluble protein. The denatured scFv was refolded and purified by immobilized metal ion affinity chromatography (12 mg/l with a molecular weight of 27 kDa). Hep27scFv exhibited a tumoricidal activity against the HCC-S102 cell as its parental antibody (Hep27 Mab). Conclusion: This scFv may be a potential candidate for a targeting agent in HCC immunodiagnosis or immunotherapy. |
| Rights: | BMC Biotechnology 2002, 2:16 This article is available from: http://www.biomedcentral.com/1472-6750/2/16 c 2002 Sandee et al; licensee BioMed Central Ltd. This article is published in Open Access: verbatim copying and redistribution of this article are permitted in all media for any non-commercial purpose, provided this notice is preserved along with the article's original URL. |
| URI: | https://hdl.handle.net/10119/8548 |
| Material Type: | publisher |
| Appears in Collections: | c10-1. 雑誌掲載論文 (Journal Articles)
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