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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/9074

Title: Mass spectrometric imaging of ginsenosides localization in Panax ginseng root
Authors: Taira, Shu
Ikeda, Ryuzo
Yokota, Naohiko
Osaka, Issey
Sakamoto, Manabu
Kato, Mitsuro
Sahashi, Yuko
Keywords: Mass spectrometry
imaging
panax ginseng
ginsenoside
localization
Issue Date: 2010
Publisher: World Scientific Publishing
Magazine name: The American Journal of Chinese Medicine
Volume: 38
Number: 3
Start page: 485
End page: 493
DOI: 10.1142/S0192415X10008007
Abstract: We succeeded in performing mass spectrometric imaging (MSI) of the localization of ginsenosides (Rb_1, Rb_2 or R_c, and Rf) in cross-sections of the Panax ginseng root at a resolution of 100 μm using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Tandem mass spectrometry (MS/MS) of alkali metal-adducted ginsenoside ions revealed structural information of the corresponding saccharides and agricones. MALDI-MSI confirmed that localization of ginsenosides in the cortex and the periderm is higher than that in the medulla of a lateral root. In addition, it revealed that localization of ginsenosides in a root tip (diameter, 2.7 mm) is higher than that in the center of the root (diameter, 7.3 mm). A quantitative difference was detected between localizations of protopanaxadiol-type ginsenoside (Rb_1、 Rb_2, or R_c) and protopanaxatriol-type ginsenoside (Rf) in the root. This imaging approach is a promising technique for rapid evaluation and identification of medicinal saponins in plant tissues.
Rights: Electronic version of an article published as The American Journal of Chinese Medicine, 38(3), 2010, 485-493. DOI:10.1142/S0192415X10008007. Copyright World Scientific Publishing Company, http://dx.doi.org/10.1142/S0192415X10008007
URI: http://hdl.handle.net/10119/9074
Material Type: author
Appears in Collections:c10-1. 雑誌掲載論文 (Journal Articles)

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