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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/9876

Title: Alzheimer's amyloid beta:lipid membrane interactions, detected in real-time
Authors: Vestergaard, Mun'delanji C.
Morita, Masumune
Hamada, Tsutomu
Takagi, Masahiro
Keywords: Amyloid beta
model membrane
Issue Date: 2010-11
Publisher: Institute of Electrical and Electronics Engineers (IEEE)
Magazine name: 2010 International Symposium on Micro-NanoMechatronics and Human Science (MHS)
Start page: 144
End page: 148
DOI: 10.1109/MHS.2010.5669562
Abstract: There are strong implications that Amyloid beta (Aβ) peptide causes neurotoxicity in Alzheimer's disease (AD) through (i) pore formation, (ii) the disruption of ionic channels that could affect calcium homeostasis, and (iii) receptor binding. The actual mechanism(s) remains unclear. In this study, we utilised cell-sized model membranes to observe the stability of lipid vesicles, in real-time, in the presence of Aβ-peptides. Using fluorescence-labelled Aβ-peptides, we imaged the localisation of pre-fibrillar Aβ species on the membrane surface, whereas mature fibrils hardly co-localised with the membrane surface. Further, we investigated the interaction of different oligomeric species of the Aβ peptide with lipid vesicles, observing membrane stability in terms of fluctuations and morphological changes. We observed a few different membrane morphological changes, with oligomeric species inducing a higher level of membrane instability. Interestingly, gramicidin A, a pore-forming peptide, did not induce any membrane transformations. We propose that this membrane transformation may be a different toxicity mechanism, from the proposed pore-formation hypothesis. This `toxicity' mechanism may aid in real-time observation of these morphological understanding the mechanisms behind Aβ-induced neuro-toxicity in Alzheimer's.
Rights: Copyright (C) 2010 IEEE. Reprinted from 2010 International Symposium on Micro-NanoMechatronics and Human Science (MHS), 2010, 144-148. This material is posted here with permission of the IEEE. Such permission of the IEEE does not in any way imply IEEE endorsement of any of JAIST's products or services. Internal or personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution must be obtained from the IEEE by writing to pubs-permissions@ieee.org. By choosing to view this document, you agree to all provisions of the copyright laws protecting it.
URI: http://hdl.handle.net/10119/9876
Material Type: publisher
Appears in Collections:c11-1. 会議発表論文 (Conference Papers)

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