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http://hdl.handle.net/10119/9876
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| タイトル: | Alzheimer's amyloid beta:lipid membrane interactions, detected in real-time |
| 著者: | Vestergaard, Mun'delanji C.
Morita, Masumune
Hamada, Tsutomu
Takagi, Masahiro |
| キーワード: | Amyloid beta
Alzheimer
model membrane
liposome
vesicle |
| 発行日: | 2010-11 |
| 出版者: | Institute of Electrical and Electronics Engineers (IEEE) |
| 誌名: | 2010 International Symposium on Micro-NanoMechatronics and Human Science (MHS) |
| 開始ページ: | 144 |
| 終了ページ: | 148 |
| DOI: | 10.1109/MHS.2010.5669562 |
| 抄録: | There are strong implications that Amyloid beta (Aβ) peptide causes neurotoxicity in Alzheimer's disease (AD) through (i) pore formation, (ii) the disruption of ionic channels that could affect calcium homeostasis, and (iii) receptor binding. The actual mechanism(s) remains unclear. In this study, we utilised cell-sized model membranes to observe the stability of lipid vesicles, in real-time, in the presence of Aβ-peptides. Using fluorescence-labelled Aβ-peptides, we imaged the localisation of pre-fibrillar Aβ species on the membrane surface, whereas mature fibrils hardly co-localised with the membrane surface. Further, we investigated the interaction of different oligomeric species of the Aβ peptide with lipid vesicles, observing membrane stability in terms of fluctuations and morphological changes. We observed a few different membrane morphological changes, with oligomeric species inducing a higher level of membrane instability. Interestingly, gramicidin A, a pore-forming peptide, did not induce any membrane transformations. We propose that this membrane transformation may be a different toxicity mechanism, from the proposed pore-formation hypothesis. This `toxicity' mechanism may aid in real-time observation of these morphological understanding the mechanisms behind Aβ-induced neuro-toxicity in Alzheimer's. |
| Rights: | Copyright (C) 2010 IEEE. Reprinted from 2010 International Symposium on Micro-NanoMechatronics and Human Science (MHS), 2010, 144-148. This material is posted here with permission of the IEEE. Such permission of the IEEE does not in any way imply IEEE endorsement of any of JAIST's products or services. Internal or personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution must be obtained from the IEEE by writing to pubs-permissions@ieee.org. By choosing to view this document, you agree to all provisions of the copyright laws protecting it. |
| URI: | http://hdl.handle.net/10119/9876 |
| 資料タイプ: | publisher |
| 出現コレクション: | c11-1. 会議発表論文 (Conference Papers)
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記述 |
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