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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/11387

Title: 核酸の生体内可視化を目指した19F 核磁気共鳴プローブの開発
Other Titles: Development of molecular probes for nucleic acids imaging based on 19F NMR/MRI
Authors: 坂本, 隆
Authors(alternative): Sakamoto, Takashi
Keywords: 分子プローブ
Issue Date: 3-Jun-2013
Abstract: 生体内で核酸を直接イメージングすることを目指し、^<19>FMR による核酸イメージングが可能な、新規分子プローブの検出感度の改善を目的に種々のフッ素ラベル剤を合成し機能評価を行った結果、(1) キラル制御された新規フッ素ラベル剤により5倍以上のS/N比の改善、(2) フッ素ラベル剤のマルチラベリングにより2.4倍の検出感度の向上、また、(3) ケミカルシフトレシオ変化型プローブの創成に成功した。 : To improve the detection limit of the probe for ^<19>F MR detection or imaging of target nucleic acids, new ^<19>F labeling reagents were synthesized and introduced to the 5’ end of oligonucleotide probe having stem-loop structure. Results of the ^<19>F NMR measurement in the presence of target DNA suggesting that the ^<19>F labels having mono chiral structure have 5-fold large S/N ratio compared with racemic one, and the multi labeling is effective for improvement of detection limit. Furthermore, the probe capable of ratiometric detection of target DNA was successfully developed.
Description: 研究種目:若手研究(B)
Language: jpn
URI: http://hdl.handle.net/10119/11387
Appears in Collections:平成24年度 (FY 2012)

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