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http://hdl.handle.net/10119/12212
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| Title: | Nucleosome Structural Changes Induced by Binding of Non-histone Chromosomal Proteins HMGN1 and HMGN2 |
| Authors: | Shimahara, Hideto
Hirano, Takaaki
Ohya, Kouichi
Matsuta, Shun
Seeram, Sailaja S.
Tate, Shin-ichi |
| Keywords: | CD
Nucleosome
HMGN
Unmodified recombinant histones
Reconstitution |
| Issue Date: | 2013-03-28 |
| Publisher: | Elsevier |
| Magazine name: | FEBS Open Bio |
| Volume: | 3 |
| Start page: | 184 |
| End page: | 191 |
| DOI: | 10.1016/j.fob.2013.03.002 |
| Abstract: | Interactions between the nucleosome and the non-histone chromosomal proteins (HMGN1 and HMGN2) were studied by circular dichroism (CD) spectroscopy to elucidate structural changes in the nucleosome induced by HMGN binding. Unlike previous studies that used a nucleosome extracted from living cells, in this study we utilized a nucleosome reconstituted from unmodified recombinant histones synthesized in Escherichia coli and a 189-bp synthetic DNA fragment harboring a nucleosome positioning sequence. This DNA fragment consists of 5′-TATAAACGCC-3′ repeats that has a high affinity to the histone octamer. A nucleosome containing a unique octamer-binding sequence at a specific location on the DNA was produced at sufficiently high yield for spectroscopic analysis. CD data have indicated that both HMGN1 and HMGN2 can increase the winding angle of the nucleosome DNA, but the extent of the structural changes induced by these proteins differs significantly. This suggests HMGN1 and HMGN2 would have different abilities to facilitate nucleosome remodeling. |
| Rights: | (c) 2013The Authors. Published by ElsevierB.V. on behalf of Federation of European Biochemical Societies. Open access under CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). Hideto Shimahara, Takaaki Hirano, Kouichi Ohya, Shun Matsuta, Sailaja S. Seeram, and Shin-ichi Tate, FEBS Open Bio, 3, 2013, 184-191, http://dx.doi.org/10.1016/j.fob.2013.03.002 |
| URI: | http://hdl.handle.net/10119/12212 |
| Material Type: | publisher |
| Appears in Collections: | g10-1. 雑誌掲載論文 (Journal Articles)
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