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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/12212

Title: Nucleosome Structural Changes Induced by Binding of Non-histone Chromosomal Proteins HMGN1 and HMGN2
Authors: Shimahara, Hideto
Hirano, Takaaki
Ohya, Kouichi
Matsuta, Shun
Seeram, Sailaja S.
Tate, Shin-ichi
Keywords: CD
Nucleosome
HMGN
Unmodified recombinant histones
Reconstitution
Issue Date: 2013-03-28
Publisher: Elsevier
Magazine name: FEBS Open Bio
Volume: 3
Start page: 184
End page: 191
DOI: 10.1016/j.fob.2013.03.002
Abstract: Interactions between the nucleosome and the non-histone chromosomal proteins (HMGN1 and HMGN2) were studied by circular dichroism (CD) spectroscopy to elucidate structural changes in the nucleosome induced by HMGN binding. Unlike previous studies that used a nucleosome extracted from living cells, in this study we utilized a nucleosome reconstituted from unmodified recombinant histones synthesized in Escherichia coli and a 189-bp synthetic DNA fragment harboring a nucleosome positioning sequence. This DNA fragment consists of 5′-TATAAACGCC-3′ repeats that has a high affinity to the histone octamer. A nucleosome containing a unique octamer-binding sequence at a specific location on the DNA was produced at sufficiently high yield for spectroscopic analysis. CD data have indicated that both HMGN1 and HMGN2 can increase the winding angle of the nucleosome DNA, but the extent of the structural changes induced by these proteins differs significantly. This suggests HMGN1 and HMGN2 would have different abilities to facilitate nucleosome remodeling.
Rights: (c) 2013The Authors. Published by ElsevierB.V. on behalf of Federation of European Biochemical Societies. Open access under CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). Hideto Shimahara, Takaaki Hirano, Kouichi Ohya, Shun Matsuta, Sailaja S. Seeram, and Shin-ichi Tate, FEBS Open Bio, 3, 2013, 184-191, http://dx.doi.org/10.1016/j.fob.2013.03.002
URI: http://hdl.handle.net/10119/12212
Material Type: publisher
Appears in Collections:f10-1. 雑誌掲載論文 (Journal Articles)

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