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http://hdl.handle.net/10119/4013
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| タイトル: | Prediction of tertiary structure of NSSRs' RNA recognition motif and the RNA binding activity |
| 著者: | Fushimi, Kazuo
Uchida, Shizuka
Matsushita, Ryousuke
Tsukahara, Toshifumi |
| キーワード: | Splicing
SR protein
Brain
Pre-mRNA
Tertiary structure
RNA recognition motif (RRM)
cDNA microarry |
| 発行日: | 2005-07 |
| 出版者: | Elsevier |
| 誌名: | Science and Technology of Advanced Materials |
| 巻: | 6 |
| 号: | 5 |
| 開始ページ: | 475 |
| 終了ページ: | 483 |
| DOI: | 10.1016/j.stam.2005.02.023 |
| 抄録: | RNAs possess potentials to become excellent bio-material because of their biochemical and biological activities. For instance, most RNA splicings are catalyzed by machinery including their own RNAs or other RNAs. The eukaryote machineries for splicing of pre-mRNA, which are called spliceosomes, are flexible and accurate for separating substrate and catalytic RNAs. Although RNAs themselves catalyze the splicing, spliceosomes are supported by many proteins. Furthermore, a great accuracy is required for the alternative splicing because there are choices available, which must be regulated in tissue-specifically and developmentally manners. Neural-salient serine/arginine-rich (NSSR) proteins 1 and 2 are candidates for supporting the accuracy of the splicing. The features of their amino acid sequences suggest that NSSRs are SR proteins, which bind to pre-mRNA and determine the splicing site. Since SR proteins have a RNA recognition motif or motives (RRM or RRMs), which binds to RNA, we predicted the secondary and tertiary structures of NSSRs' RRM by comparing them to RRMs of other proteins. The predicted structure suggested that the RNA binding activity of NSSRs' RRM is similar to the poly A binding protein (PABP). Moreover, to detect the targets for NSSR, mRNAs were obtained by screening them from murine brains with bacterial recombinant NSSRs' RRM and microarray experiments were conducted using these mRNAs. The results suggested that NSSRs bind specifically to particular pre-mRNAs and regulate the alternative splicing of the binding pre-mRNA. |
| Rights: | Elsevier Ltd, Kazuo Fushimi, Shizuka Uchida, Ryousuke Matsushita and Toshifumi Tsukahara, Science and Technology of Advanced Materials, 6(5), 2005, 475-483. http://www.science-direct.com/science/journal/14686996 |
| 資料タイプ: | Article |
| URI: | http://hdl.handle.net/10119/4013 |
| 資料タイプ: | author |
| 出現コレクション: | g10-1. 雑誌掲載論文 (Journal Articles)
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