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Please use this identifier to cite or link to this item: http://hdl.handle.net/10119/4014

Title: NSSRs/TASRs/SRp38s function as splicing modulators via binding to pre-mRNAs
Authors: Fushimi, Kazuo
Osumi, Noriko
Tsukahara, Toshifumi
Keywords: Alternative splicing
SR protein
Differentiation
Neuron
Brain
MS2 phage
Issue Date: 2005-06
Publisher: Blackwell Science
Magazine name: Genes to Cells
Volume: 10
Number: 6
Start page: 531
End page: 541
DOI: 10.1111/j.1365-2443.2005.00855.x
Abstract: The genes for neural-salient serine/arginine-rich (NSSR) proteins 1 and 2 have been cloned from a neuronal differentiated embryocarcinoma cell line, P19. NSSRs contain an RNA recognition motif (RRM) at the N-terminal and several SR rich regions at the C-terminal resembling RS domains. We found that NSSRs associated with U1-70k, and determined the exon inclusion activity of NSSRs' C-terminals. First, the RRM was changed to the MS2 coat protein (MS2CP) and then, MS2 RNA stem-loops were inserted in the middle of the exon N of the clathrin light chain B minigene as an artificial exonic splicing enhancer to be recognized by the MS2CP. The modified exon N of the pre-mRNA was included by the MS2CP switched NSSR 1, but it was excluded by the MS2CP switched NSSR 2. Deletion analysis of the MS2CP switched NSSR1 suggested that the middle SR rich region was responsible for the activity of the modified exon N inclusion. Furthermore, the RRM domain of NSSRs recognized mRNAs. NSSRs were expressed in the nervous system, especially in cerebellar and hippocampal primordia, neopallial cortex, ventricular zone, retina, and olfactory epithelium and bulb at E15.5. Taken together, our results showed that NSSRs modulate alternative splicing via binding to pre-mRNAs during neural differentiation.
Rights: BLACKWELL SCIENCE INC, Fushimi, Kazuo ; Osumi, Noriko ; Tsukahara, Toshifumi, Genes to Cells, 10(6), 2005, 531-541. The definitive version is available at www.blackwell-synergy.com
Type: Article
URI: http://hdl.handle.net/10119/4014
Material Type: author
Appears in Collections:g10-1. 雑誌掲載論文 (Journal Articles)

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