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このアイテムの引用には次の識別子を使用してください:
http://hdl.handle.net/10119/4014
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| タイトル: | NSSRs/TASRs/SRp38s function as splicing modulators via binding to pre-mRNAs |
| 著者: | Fushimi, Kazuo
Osumi, Noriko
Tsukahara, Toshifumi |
| キーワード: | Alternative splicing
SR protein
Differentiation
Neuron
Brain
MS2 phage |
| 発行日: | 2005-06 |
| 出版者: | Blackwell Science |
| 誌名: | Genes to Cells |
| 巻: | 10 |
| 号: | 6 |
| 開始ページ: | 531 |
| 終了ページ: | 541 |
| DOI: | 10.1111/j.1365-2443.2005.00855.x |
| 抄録: | The genes for neural-salient serine/arginine-rich (NSSR) proteins 1 and 2 have been cloned from a neuronal differentiated embryocarcinoma cell line, P19. NSSRs contain an RNA recognition motif (RRM) at the N-terminal and several SR rich regions at the C-terminal resembling RS domains. We found that NSSRs associated with U1-70k, and determined the exon inclusion activity of NSSRs' C-terminals. First, the RRM was changed to the MS2 coat protein (MS2CP) and then, MS2 RNA stem-loops were inserted in the middle of the exon N of the clathrin light chain B minigene as an artificial exonic splicing enhancer to be recognized by the MS2CP. The modified exon N of the pre-mRNA was included by the MS2CP switched NSSR 1, but it was excluded by the MS2CP switched NSSR 2. Deletion analysis of the MS2CP switched NSSR1 suggested that the middle SR rich region was responsible for the activity of the modified exon N inclusion. Furthermore, the RRM domain of NSSRs recognized mRNAs. NSSRs were expressed in the nervous system, especially in cerebellar and hippocampal primordia, neopallial cortex, ventricular zone, retina, and olfactory epithelium and bulb at E15.5. Taken together, our results showed that NSSRs modulate alternative splicing via binding to pre-mRNAs during neural differentiation. |
| Rights: | BLACKWELL SCIENCE INC, Fushimi, Kazuo ; Osumi, Noriko ; Tsukahara, Toshifumi, Genes to Cells, 10(6), 2005, 531-541. The definitive version is available at www.blackwell-synergy.com |
| 資料タイプ: | Article |
| URI: | http://hdl.handle.net/10119/4014 |
| 資料タイプ: | author |
| 出現コレクション: | g10-1. 雑誌掲載論文 (Journal Articles)
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| S-241.pdf | | 470Kb | Adobe PDF | 見る/開く |
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